Objective
@EN This study was performed to evaluate the prostaglandin secretion rates in human umbilical vein with preeclamptic sera and peroxide perfusion.
@ES Study design
@EN Isolated human umbilical cords(n=10) were perfused for 30-minute intervals with cord buffer, 15% normal pregnant sera and preeclamptic patient sera, 100 mol/L t-butyl hydroperoxide alone, and after perfusion with low-dose aspirin(5 x 10E-5
mol/L).
Cord buffer gassed with 95% oxygen and 5% carbon dioxide and warmed to 37℃ was used for the perfusion buffer. Effluent flow rates were measured during each experimental treatment. Effluent samples were measured for 6-keto prostaglandin F1α and
thromboxane B2 by enzyme immunoassays.
@ES The concentrations of 6-keto prostaglandin F1α in preeclamptic sera were significantly higher than those in normal pregnant sera. (989.3849±1602.927 vs. 1.3116±1.22085 ng/ml, mean ± SD, p<0.01). However, the concentrations of thromboxane
B2
were
not different between normal pregnant sera and preeclamptic sera. The secretion rate of 6-keto-prostaglandin F1α in human umbilical endothelial cells w as not significantly different(p=0.77) between two groups. comparing to normal pregnant sera,
the
secretion rate of thromboxane B2 was significantly increased(p<0.01) after preeclamptic sera perfusion. The secretion rate of 6-keto-prostaglandin F1 was significantly increased(p<0.01) following peroxide perfusion and that was significantly
decreased
by aspirin. The secretion rate of thromboxane B2 was not significantly different between preeclamptic sera and peroxide alone or subsequent perfusion with aspirin.
@ES Conclusions
@EN (1) Preeclamptic sera stimulate thromboxane production rather than prostacyclin production by endothelial cells of human umbilical vein in vitro. (2) Peroxide stimulates the secretion of both prostacyclin and thromboxane, and low dose aspirin
mitigates hydroperoxide-induced prostacyclin secretion. We confirmed that thromboxane secretion is stimulated by preeclamptic sera and the role of peroxide in prostaglandin secretion. We established the perfusion system using human umbilical vein
through this study. This perfusion system be useful to understand the pathophysiology of preeclampsia.
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